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Microscopy technique

Slides, objectives, magnification, measurement and the things on a slide that are not spores.

Close-up of compound microscope objectives over a slide
400x is the working magnification. 1000x with oil is where the detail lives.

The practical craft of spore microscopy: preparing a wet mount, choosing a compound microscope, knowing what 400x and 1000x each show, measuring a population against a graticule, reading a spore print and recognising bacteria, moulds and yeast when they turn up. Each guide is built around Psilocybe cubensis as the reference and the other species in the library as comparisons.

A hand placing a glass slide beneath a microscope objectiveMicroscopy techniqueNo. 12

How to view mushroom spores under a microscope: slide prep, magnification and what to record

A practical guide to spore microscopy: preparing a wet mount from a spore syringe, which magnification to use, measuring spores with a graticule, and what to write in your notebook.

20 September 2026 · 2 min read · Read →
Close-up of a compound microscope's objective lenses over a slideMicroscopy techniqueNo. 13

Choosing a microscope for spore study in the UK: what matters and what does not

What microscope do you need to study mushroom spores? Compound not stereo, 400x as the working magnification, a 100x oil objective for detail, and the features worth paying for.

20 September 2026 · 2 min read · Read →
Psilocybe pelliculosa spores at high magnification with a 10 micrometre scale barMicroscopy techniqueNo. 14

What magnification do you need to see mushroom spores?

Mushroom spores are visible at 100x, clear at 400x and fully resolved at 1000x with oil. What each magnification shows, why more is not better, and how to measure what you see.

20 September 2026 · 2 min read · Read →
Psilocybe stuntzii spores at 1000x magnificationMicroscopy techniqueNo. 15

Psilocybe cubensis spore morphology: shape, size, wall and germ pore under the microscope

What Psilocybe cubensis spores look like at 400x and 1000x: subellipsoid, 11 to 17 micrometres, thick-walled with a germ pore, purple-brown in mass. How to measure and record them and how strains differ.

20 September 2026 · 2 min read · Read →
A round brown spore print on white paperMicroscopy techniqueNo. 16

Spore print colour: what purple-brown, black, white and pale grey tell you

Spore print colour is a primary taxonomic character. What purple-brown, black, rusty, white and pale grey deposits mean, how to read density and pattern, and how to store a print.

20 September 2026 · 2 min read · Read →
Grey-green Penicillium mould growing on a red surfaceMicroscopy techniqueNo. 17

Contamination under the microscope: telling bacteria, mould and debris from spores

How to recognise bacteria, mould spores, yeast and debris on a spore slide, what a contaminated syringe looks like, and how sterile preparation keeps a specimen clean.

20 September 2026 · 2 min read · Read →
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Specimens for this chapter

The strains these stories talk about, when they are in stock, prepared under sterile protocol and posted from the UK.

Every specimen on MycoWorld is supplied strictly for microscopy, taxonomy and research. Nothing here is a cultivation product and we do not provide or endorse cultivation guidance. 18+ only. Read the Legal Use Policy.

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